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MSC-derived EVs were retained in the poststenotic kidney. Fragments of red immunofluorescent stained EVs (PKH26, arrows; original magnification ×40) were detected in the stenotic kidney of MetS+RAS+Lean-EVs and MetS+RAS+MetS-EVs 4 weeks after administration, but not in untreated MetS+RAS stenotic kidneys. Immunofluorescent costaining with cytokeratin identified EV fragments in the vicinity of renal tubular cells, whereas some of them colocalized with renal tubular cells.
