TABLE 1.

Growth conditions for filamentous fungi generating the most distinguishable peaks^a

Growth conditions	Method for harvest of material	Extraction step(s)	Species (no. of isolates)	Reference
72 h at 27°C on Sabouraud gentamicin-chloramphenicol agar plates	Harvested by scraping with a sterile plastic device	FA extraction	Aspergillus spp. (43), Penicillium spp. (16), Scedosporium spp. (18), Fusarium spp. (11), other (68)	1
24 h at 27°C in Sabouraud broth	10-min centrifugation at 13,000 × g	Pellet washed 3 times with 1 ml of sterile water and suspended with 300 μl of HPLC sterile water and 900 μl of anhydrous ethyl alcohol; FA extraction	Aspergillus spp. (43), Penicillium spp. (16), Scedosporium spp. (18), Fusarium spp. (11), other (68)	1
72 h at 27°C on Sabouraud gentamicin-chloramphenicol agar plates	Harvested by scraping with a sterile plastic device	Hydroalcoholic suspension of fungal material lysed by 3 cycles of microbeads with a FastPrep-24 instrument; FA extraction	Aspergillus spp. (43), Penicillium spp. (16), Scedosporium spp. (18), Fusarium spp. (11), other (68)	1
72 h at 27°C on Sabouraud dextrose agar with antibiotics		FA extraction	Pseudallescheria-Scedosporium species complex	8
72 h at 27°C on malt agar		TFA extraction	Pseudallescheria-Scedosporium species complex	8
72 h at 27°C on malt agar	Surfaces scraped using a sterile scalpel	TFA extraction	Clinical Fusarium isolates	9

^aAbbreviations: FA, formic acid; HPLC, high-performance liquid chromatography; TFA, trifluoroacetic acid.