TABLE 1.
Mycoplasma inoculation groups (10 pigs per group)a
| Study | Inoculum | Route of inoculation (dose [ml]) | Concnb |
|---|---|---|---|
| 1 | Negative control (Friis media) | Intranasal (0.5/nostril) | NA |
| 1 | M. flocculare strain 27399 | Tonsil of the soft palate (2) | 1.0 × 105 CCU/ml |
| Intranasal (1) | 1.0 × 105 CCU/ml | ||
| Intratracheal (1) | 1.0 × 105 CCU/ml | ||
| 1 | M. hyorhinis strain 38983 | Tonsil of the soft palate (2) | 3.2 × 108 CFU/ml |
| Intraperitoneal (2) | 3.2 × 108 CFU/ml | ||
| 1 | M. hyosynoviae strain 34428 | Tonsil of the soft palate (2) | 2.1 × 109 CFU/ml |
| Intranasal (0.5/nostril) | 2.1 × 109 CFU/ml | ||
| Intravascular (1 ml/ear vein) | 2.1 × 109 CFU/ml | ||
| 1 | M. hyopneumoniae strain 232c | Intratracheal (1 ml) | 1.0 × 106 CCU/ml |
| 2 | Intratracheal (10 ml) | 1.0 × 105 CCU/ml |
a
Table adapted from reference 14.
b
The purity of original seeds and final inoculum was evaluated by qPCR and microscopy staining (×1,000 magnification) to rule out bacterial contamination, including other Mycoplasma spp.
c
M. hyopneumoniae strain 232 inoculum was lung tissue from an infected pig homogenized and diluted 1:100 in Friis media.