TABLE 3.
JD-infected herds screened by RL-PCR and ELISA in this study
| Herd ID | Herd type | No. of animals screened | Screened by RL-PCR |
Screened by ELISA |
|||||
|---|---|---|---|---|---|---|---|---|---|
| % of positive pools | No. of positive animals | No. of SYBRa-positive animals (no. diagnosed) | Prevalence (%)b | No. of positive animals | No. of SYBRa-positive animals (no. diagnosed) | Prevalence (%)b | |||
| P-1 | Dairy | 90 | 11.1 | 1 | 1 (1) | 1.11 | 6 | 0 | 0 |
| P-2 | Dairy | 246 | 4.0 | 1 | 1 (1) | 0.41 | 0 | 0 | 0 |
| P-3 | Dairy | 386 | 100 | 265 | 243 (18) | 4.66 | 4 | 3 (1) | 0.26 |
| P-4 | Dairy | 133 | 35.7 | 10 | 7 (0) | 0 | 2 | 0 | 0 |
| P-5 | Dairy | 88 | 22.2 | 2 | 1 (0) | 0 | 0 | 0 | 0 |
| P-6 | Dairy | 112 | 75.0 | 21 | 17 (5) | 4.46 | 3 | 2 (1) | 0.89 |
| P-7 | Dairy | 498 | 10.0 | 6 | 6 (4) | 0.80 | 0 | 0 | 0 |
| P-8 | Dairy | 335 | 60.0 | 35 | 22 (9) | 2.69 | 1 | 1 (1) | 0.30 |
| P-9 | Dairy | 255 | 53.8 | 23 | 17 (10) | 3.92 | 1 | 1 (1) | 0.39 |
| P-10 | Dairy | 150 | 26.7 | 20 | 17 (0) | 0 | 0 | 0 | 0 |
| P-11 | Beef | 149 | 26.7 | 6 | 6 (2) | 1.34 | 2 | 1 (1) | 0.67 |
| P-12 | Beef | 212 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
| Total | 2,654 | 390 | 338 (50) | 20 | 8 (5) | ||||
a
Conventional SYBR green-based qPCR assay.
b
Herd prevalence of JD based on the number of diagnosed animals detected through the screening test.