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Effect of pooling on the sensitivity of RL-PCR in qPCR-positive feces
| Pooled RL-PCRa | Values by DNA quantity (pg/well)b
of: |
Total | |
|---|---|---|---|
| <0.001 | ≥0.001 | ||
| No. tested | 23 | 41 | 64 |
| No. positive (%) | 15 (65.2) | 39 (95.1) | 54 (84.4) |
Tested at a pool size of 10 (1 positive plus 9 negative fecal samples).
DNA quantity detected by the conventional SYBR green-based qPCR assay.
