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Workflow and analysis of automated 9× and automated 1×, concentrated (A) and iSCA v2 (B). Three methods to quantify low-level HIV-1 RNA in plasma of HIV-1-positive but virologically suppressed individuals were compared. Two methods use the Hologic Panther Aptima automated platform (A) and the third uses a published manual single-copy assay (iSCA v2) (B).
