TABLE 1.

Summary of HIV-1 RNA quantification and variance by assay method

Methoda	Expected concn (cps/ml)	Mean (± SD) observed concn (cps/ml)	%CV	Mean %CV
Automated 9×	20.0	6.08 ± 1.93	31.7	33.6
	5.0	1.27 ± 0.63	49.6
	2.5	0.87 ± 0.31	35.6
	1.2	0.55 ± 0.16	29.1
	0.6	0.36 ± 0.08	22.2
Automated 1×, concentrated	20.0	11.70 ± 2.56	21.9	35.8
	5.0	1.70 ± 0.58	34.1
	2.5	1.04 ± 0.57	54.8
	1.2	0.33 ± 0.13	39.4
	0.6	0.14 ± 0.04	28.6
iSCA v2	20.0	17.12 ± 16.40	95.8	68.4
	5.0	3.52 ± 1.92	54.5
	2.5	1.30 ± 0.90	69.2
	1.2	1.0 ± 0.60	60
	0.6	0.80 ± 0.50	62.5

^aA total of 25 replicates were run for each concentration and method.