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. 2020 Nov 18;58(12):e01972-20. doi: 10.1128/JCM.01972-20

TABLE 6.

Sanger versus nanopore R9.4.1 flow cell sequence IGS mismatches

Strain Genotype No. of errors
% identityb
Homopolymer mismatches
Repetitive motifs Deletionsa Mismatches
A T G C Total
W3482 VGI 0 0 0 0 0 0 0 0 100
W10 VGI 1 7 6 0 14 0 0 0 99.55
W85 VGI 3 4 5 0 12 0 0 0 99.64
W3481 VGII 4 4 4 0 12 0 0 0 99.52
W432 VGII 1 5 4 0 10 0 0 0 99.58
W530 VGII 5 5 5 0 15 2 0 0 99.38
W3479 VGIII 0 4 5 0 9 0 0 0 99.73
W15 VGIII 1 0 3 0 4 0 0 0 99.85
W16 VGIII 3 5 5 0 13 0 0 1 99.58
W83 VGIII 3 3 6 0 12 0 0 0 99.61
W84 VGIII 0 4 2 0 6 0 0 0 99.76
W87 VGIII 2 4 4 0 10 0 0 0 99.70
W89 VGIII 2 3 4 0 9 0 0 0 99.70
W90 VGIII 1 4 3 0 8 0 0 0 99.70
W3480 VGIV 1 5 5 0 11 0 0 0 99.61
W3477 VNI 6 3 8 0 17 0 0 0 99.48
W72 VNI 5 2 5 0 12 0 0 0 99.63
W473 VNI 6 4 6 0 16 0 0 1 99.51
W516 VNI 5 4 7 0 16 5 0 6 99.20
W1047 VNI 7 3 10 0 20 0 1 1 99.34
H99 VNI 6 3 7 0 16 2 0 2 99.23
W3478 VNII 7 3 9 0 19 0 0 0 99.34
W3484 VNIV 1 4 8 0 13 2 0 0 99.48
JEC21 VNIV 1 5 8 0 14 3 0 0 99.48
W77 VNIV 3 4 4 0 11 0 0 0 99.54
W78 VNIV 1 0 2 1 4 2 0 0 99.75
W79 VNIV 2 3 4 0 9 2 0 0 99.60
Total 77 95 139 1 301 18 1 11
Avg 2.85 3.52 5.15 0.04 11.15 0.67 0.04 0.41 99.57
a

Deletions in regions other than a homopolymer.

b

Based on comparison to Sanger sequence of the same region.