TABLE 8.

Sanger versus nanopore R10.3 flow cell sequence IGS mismatches

Strain	Genotype	No. of errors								% identityb
		Homopolymer mismatches					Repetitive motifs	Deletionsa	Mismatches
		A	T	G	C	Total
W3482	VGI	0	0	2	1	3	0	0	0	99.91
W10	VGI	1	1	3	0	5	0	0	0	99.85
W85	VGI	0	0	2	0	2	0	0	0	99.94
W3481	VGII	0	2	6	1	9	0	0	0	99.73
W432	VGII	0	1	5	0	0	6	0	0	99.82
W530	VGII	1	1	6	0	8	0	2	0	99.70
W3479	VGIII	0	0	1	1	2	0	0	0	99.94
W15	VGIII	1	0	3	0	4	0	0	0	99.88
W16	VGIII	1	0	3	0	4	0	0	1	99.85
W83	VGIII	0	1	2	0	3	0	0	0	99.91
W84	VGIII	2	2	4	0	8	2	0	0	99.70
W87	VGIII	0	1	2	0	3	0	0	0	99.91
W89	VGIII	0	1	3	1	5	0	0	0	99.85
W90	VGIII	2	1	3	0	6	0	0	0	99.82
W3480	VGIV	0	0	2	0	2	0	0	0	99.94
W3477	VNI	0	1	7	0	8	2	0	0	99.71
W72	VNI	1	0	1	1	3	0	0	0	99.91
W473	VNI	2	0	2	0	4	0	0	1	99.86
W516	VNI	0	0	2	0	2	2	0	0	99.89
W1047	VNI	2	0	1	0	3	0	0	0	99.91
H99	VNI	1	0	3	0	4	2	0	0	99.83
W3478	VNII	5	0	4	0	9	0	0	0	99.74
W3484	VNIV	6	0	3	0	9	2	0	2	99.60
JEC21	VNIV	0	0	2	1	3	0	1	0	99.87
W77	VNIV	6	0	2	0	8	2	0	0	99.70
W78	VNIV	4	0	2	1	7	0	0	0	99.79
W79	VNIV	1	0	2	1	4	0	0	0	99.88
Total		36	12	78	8	128	18	3	4
Avg		1.33	0.44	2.89	0.30	4.74	0.67	0.11	0.15	99.83

^aDeletions in regions other than a homopolymer.

^bBased on comparison to Sanger sequence of the same region.