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. 2020 Mar 17;34(12):3242–3255. doi: 10.1038/s41375-020-0789-x

Fig. 1. ALCL stem cells derive from CD4−/CD8− double negative lymphoma T cells.

Fig. 1

a A Kaplan–Meier survival curve of primary transplanted mice. 50,000 EGFP positive MSNAIE Lck-Cre transgenic or wildtype BM cells were injected i.v. into lethally irradiated (8500 rad) C57Bl6 recipient mice. Median survival: 130 days. n (wildtype) = 15, n (Lck-Cre) = 22. b Comparison of spleen and thymi weights of control (n = 9) and MSNAIE Lck-Cre transplanted mice (n = 15). c Flow cytometric dot plots of EGFP positive cells in the thymus of one representative MSNAIE Lck-Cre BM transplanted mouse stained with anti-B220, anti-GR1, anti-CD11b, anti-Thy1.2, anti-CD4, and anti-CD8. d Flow cytometric dot plots of lymphatic organs and BM of primary MSNAIE Lck-Cre BM transplanted mice stained for anti-CD4, anti-CD8, and TCR α/β. e Sorting strategy for secondary transplantation of EGFP positive T cell subpopulations. Single cell suspensions of the thymus were stained with anti-CD4 and anti-CD8 and sorted for the four T cell subpopulations. f Kaplan–Meier survival curve of secondary transplanted mice. 2500 EGFP positive lymphoma cells (CD4/CD8 subpopulation) were injected i.v. into sublethally irradiated (4500 rad) C57Bl6 recipient mice. Median survival of CD4−/CD8− transplanted mice: 68 days n = 6. g White blood counts (WBC) at death of peripheral blood isolated from CD4−/CD8− transplanted recipients or at day 120 in case of the other CD4/8 subgroup. Six mice in each subgroup were analysed. h WBC counts over time of Tx of serial CD4/CD8 lymphoma subpopulation transplanted mice. Six mice in each subgroup were analysed. i Comparison of spleen weights of serial CD4/CD8 lymphoma subpopulation transplanted mice at death (CD4−/CD8−) or at the end of Tx (other subgroups). Six mice in each subgroup were analysed. ***p < 0.001. Data are represented as means ± SD.