Fig. 3. Primary MSNAIE Lck-Cre induced lymphomas contain a heterogeneous cell population ranging from undifferentiated hematopoietic stem cells to mature T cells.

a Representative flow cytometry profiles of EGFP⁺ thymus and BM cells of MSNAIE Lck-Cre transgenic BM transplanted mice. Cells were stained for anti-CD4, anti-CD8, anti-CD25, and anti-CD44. b Representative flow cytometry profiles of EGFP⁺ lineage negative (lin-) thymus and BM cells of MSNAIE Lck-Cre transgenic BM transplanted mice. Cells were stained for hematopoetic stem cell and lymphatic precursor proteins anti-SCA1, anti-cKIT, and anti-IL7Rα. LSK: lineage⁻SCA1⁺cKIT⁺; MP multipotent progenitors, lineage⁻SCA1⁻cKIT⁺; CLP common lymphoid progenitors, lineage⁻SCA1^lowcKIT^lowIl7Rα⁺. c Pie chart of EGFP⁺ T cell subpopulations differentiated for CD4 and CD8 (left charts) of thymus and BM of five independent MSNAIE Lck-Cre transgenic BM transplanted mice. Distribution over the different cell types within the EGFP⁺ CD4⁻/CD8⁻ DN lymphoma subpopulation are shown from five independent diseased animals on the right. CLP common lymphoid progenitors, lineage⁻SCA1^lowcKIT^lowIl7Rα⁺, LSK lineage⁻SCA1⁺cKIT⁺, MP multipotent progenitors, lineage⁻SCA1⁻¹⁶⁰cKIT⁺.