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. 2020 Mar 17;34(12):3242–3255. doi: 10.1038/s41375-020-0789-x

Fig. 4. Lymphoma stem cells reside within the DN3/DN4 T cell population, but not the DN1/DN2 population.

Fig. 4

a Strategy for secondary transplantation of lymphoma subpopulations: 1500 EGFP positive CD4−/CD8− double negative lymphoma T cells of stages DN1 to DN4 were injected i.v. into subl-ethally irradiated (4500 rad) C57Bl6 recipient mice. b Kaplan–Meier survival curve of serially transplanted mice. Median survival: DN3 transplanted mice: 41 days, DN4 transplanted mice: 50 days. Representative flow cytometry profiles of lymphatic organs, thymus (Thy), spleen (Spl), lymph node (LN) and bone marrow (BM) of DN3 (c, e) and DN4 (d) transplanted mice stained for anti-CD4, anti-CD8, anti-CD44, and anti-CD25. *p < 0.05; ***p < 0.001.