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. 2020 Jun 19;34(12):3323–3337. doi: 10.1038/s41375-020-0901-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Total number of whole bone marrow cells is reduced in S100A6KO mice. b Lineage distribution in steady-state peripheral blood. c S100A6KO HSCs have significant higher levels of apoptotic cells. d Schematic gating of LSK, CD150, CD48 HSPC compartment, in steady-state whole bone marrow cells. Representative FACS plots of LT-HSC (CD150⁺CD48⁻) and multipotent progenitor (MPP, CD150⁻CD48⁻). S100A6KO had a robust reduction in the LT-HSC and MPP compartments compared with WT. e, f Histogram summarized total cells number within the LT-HSC and MPP in bone marrow. g C-kit-enriched FACS sorted bone marrow cells (LSK CD150⁺CD48⁻CD34⁻Flt3⁻). h More stringent gating of FACS sorted c-kit-enriched bone marrow cells (LSK CD150⁺CD48⁻ CD34⁻Flt3⁻ESAM⁺CD9^Hi) (*p < 0.05; **p < 0.001; ***p < 0.0001; all data were analyzed by unpaired two-sided t-test and Mann–Whitney U test). All individual dots represent randomized biological replicates with matched littermates and experiments were repeated three times.