Fig. 6. The LT-HSCs in S100A6KO mice were unable to respond to 5FU chemotoxic stress and the chaperonin Hsp90 activity of S100A6KO was restored by Akt activator.

a Frequencies of LT-HSC (LSKMac^lo/Mac⁻CD150⁺CD48⁻) in total bone marrow 12 days after 5FU administration (WT n = 3, KO n = 5). b Frequencies of LT-HSC (LSKMac⁻CD150⁺CD48⁻) in total bone marrow 12 days after 5FU administration (WT n = 4, KO n = 5). c, d Frequencies and total number of cells from a stringent gating of LT-HSCs (LSKMac⁻CD150⁺CD48⁻ESAM⁺CD9^Hi) in c-kit-enriched bone marrow cells 12 days after 5FU administration. Steady state (SS), stress introduced with 5FU administration (S) (SS (WT, KO) n = 4; S (WT, KO) n = 3). e Absolute numbers of total c-kit-enriched bone marrow cells 12 days after 5FU administration (SS (WT, KO) n = 4; S (WT, KO) n = 3) (*p < 0.05; **p < 0.001; *** or ****p < 0.0001; all data dissected by unpaired two-sided t-test and Mann–Whitney U test). f Intracellular staining of Hsp40 in LT-HSCs (n = 3). g Intracellular staining of Hsp90 in LT-HSCs. Akt activator, SC79 restored Hsp90 activity in the KO (f, g) (n = 3). Data represent mean values from independent experiments ± SD. *p < 0.05; **p < 0.001, gated on CD150⁺CD48⁻CD34⁻Flt⁻, analyzed by unpaired two-sided t-test. The variance found inside values in a single group was smaller than the variance caused by interactions between different samples (one-way ANOVA, p = 0.006).