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. 2020 Nov 11;7:585276. doi: 10.3389/fvets.2020.585276

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Copyright © 2020 Xu, Xie, Zhou, Hu, Gu, Yang, Zheng, Huang, Xu, Cai, Liu, Wu and Hong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The design of luciferase reporter. VCL 3' UTR sequence contains the ssc-miR-21-5p binding site; VCL 3' UTR Mut sequence contains mutation of the ssc-miR-21-5p binding site. (B) Luciferase activity was analyzed 48 h after co-transfection of PK15 cells with VCL3' UTR (WT) or VCL 3' UTR Mut plasmid (Mut) and ssc-miR-21-5p mimics (miR-21) or mimics negative control (miR-NC). pGL3 used as the basic vector of the luciferase reporter. (C) The ssc-miR-21-5p binding site sequences on the VCL 3′ UTR is conserved across species.