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. 2020 Nov 11;11:582577. doi: 10.3389/fpls.2020.582577

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Copyright © 2020 Wang, Severing, Koornneef and Aarts.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Variation in vernalization response and NcFLC expression of selected N. caerulescens accessions. (A) The transition to flowering as determined by the time to bolting (first flower bud visible) for non-vernalized (NV) plants and for plants vernalized for the indicated number of weeks (W). (B) The time to flowering (first petal visible); and (C) the number of inflorescences per plant in the same treatments as (A). (D) The relative expression of NcFLC in rosette leaves upon vernalization for the indicated number of weeks, relative to the expression of NcFLC in SF after 12 weeks of vernalization (=1). The expression of NcTubulin is used to normalize cDNA concentrations. Values are the mean of three to five plants. Bars show standard errors. NV-1: 2-month-old non-vernalized plants; NV-2: 5-month-old non-vernalized plants. NB, not bolting; NF, not flowering. Accessions are Ganges (GA), St. Felix de Pallières (SF), Lellingen (LE), and La Calamine (LC).