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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Biochem Pharmacol. 2020 Oct 10;182:114280. doi: 10.1016/j.bcp.2020.114280

Fig. 6.

Fig. 6.

Transient expression of FLAG-DDX3 proteins. (A–D) Characterization of exogenous DDX3-FLAG on G3BP1-dependent SG assembly upon 90 min of 0.5 mM arsenite in U2OS cells with anti-G3BP1 (red) and anti-FLAG (green). Scale bar, 10 µm. (A) Vector control with G3BP1 and FLAG. Overexpressed (B) wild-type, (C) ATPase-site mutant, and (D) Helicase-site mutant with G3BP1 and FLAG. The full arrows represent localization of G3BP1 with overexpressed DDX3-FLAG; the arrowheads represent endogenous G3BP1-dependent SGs.