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IF of OCT4 (ICM marker), active TRP53 (apoptosis marker), and CDX2 (TE marker) in E3.0 morulae of different genotypes. None of the embryos showed obvious TRP53-positive cells in all genotypes. OCT4 expression was comparable in Polr1d mutants, CDX2 expression was strikingly decreased when compared with WT and Het littermates. Scale bar, 50 μm.
