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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Mol Reprod Dev. 2020 Oct 6;87(11):1152–1158. doi: 10.1002/mrd.23427

Figure 4.

Figure 4.

(A) Fluorescent whole-mount TUNEL assay was performed to evaluate if POLR1D is involved in DNA damage and cell death. (B) IF of ROS (marker for oxidative homeostasis) in embryos of different genotypes. All genotypes displayed similarly low ROS levels, suggesting that embryonic lethality of Polr1d mutants is not due to oxidative stress, nor does POLR1D significantly regulate oxidative homeostasis during early embryo development in mice. Scale bars, 50 μm.