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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Ocul Surf. 2020 Aug 13;18(4):821–828. doi: 10.1016/j.jtos.2020.07.021

Figure 1. Cervical dislocation and delayed fixation by 5 min after euthanasia do not impact intraepithelial corneal nerve density (ICNs).

Figure 1.

A. A representative en face image of a flat mounted mouse cornea stained to visualize the ICNs is shown. Axon density is assessed by Sholl analysis by placing targets at 7 sites: four at the corneal periphery and 3 at the corneal center. Bar = 500 μm. B. Axon density was assessed by Sholl analysis and the means and standard error of the means assessed for 10 corneas. Our standard of care (SOC, CO2 gas) was compared to cervical dislocation (CD) alone and CD after isoflurane gas to anesthetize mice (ISO). In addition, we determined whether delaying enucleation for 5 minutes followed by fixation of the eyes after SOC euthanasia or CD impacts axon density. No significant differences were observed.