FIGURE 3.
HIV‐1 infection attenuates cav‐1‐mediated regulation of ocln expression. Pericytes were transfected with 1 µg cav‐1 siRNA per 106 cells and either mock‐infected or HIV‐1‐infected with 60 ng/mL HIV‐1 p24 for 48 h. The expression of cav‐1, ocln, and Alix was evaluated by immunoblotting (A), quantified, and compared among groups. GAPDH was used as a loading control. Cav‐1 silencing (B) resulted in upregulation of occludin levels in mock‐infected pericytes and this effect was attenuated in HIV‐1‐infected pericytes (C). Cav‐1 silencing did not affect Alix levels (D). Graphs indicate the mean ± SD from three independent experiments. ****P < .0001, ***P = .0002, **P = .003, n = 4‐9 per group