FIGURE 4.
HIV‐1 infection obliterates ocln‐mediated regulation of cav‐1 expression. Pericytes were transfected with 2 µg ocln expression vector per 106 cells and either mock‐infected or HIV‐1‐infected with 60 ng/mL HIV‐1 p24 for 48 h. The expression of ocln, cav‐1, and Alix was evaluated by immunoblotting (A) and compared among groups. Tubulin was used as a loading control. Ocln overexpression (B) resulted in downregulation of cav‐1 in mock‐infected pericytes and this effect was obliterated in HIV‐1‐infected pericytes (C). Ocln overexpression did not affect Alix levels (D). Graphs indicate the mean ± SD from three independent experiments. ****P < .0001, ***P = .0002, n = 4‐9 per group