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. Author manuscript; available in PMC: 2021 Nov 23.
Published in final edited form as: Dev Cell. 2020 Oct 14;55(4):468–482.e7. doi: 10.1016/j.devcel.2020.09.022

Figure 6. LIM proteins bind along filament regions featuring a pre-break state of F-actin.

Figure 6.

(A) Montages of indicated constructs forming patches in the middle (top) and at the terminal segments (bottom) of actin filaments in the presence of force generation. Scale bars, 2 μm. (B) Kymograph (left, FHL3 channel) and montage (right) of an FHL3 patch. Snapshots correspond to the time points indicated along the kymograph. Scale bar, 2 μm. (C) Intensity line scan along dotted lines shown in B before (top) and after (bottom) FHL3 disappearance. (D) Log ratio histograms of actin intensity at detected patches of the indicated constructs before versus immediately after LIM-protein disappearance. Dashed line denotes equal intensity. See also Figures S5 and S6.