KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-FHL2 | Abcam | Cat# ab202584 |
| Mouse monoclonal anti-paxillin | BD Biosciences | Cat# 610052 |
| Alexa Fluor 488 goat anti-rabbit secondary antibody | ThermoFisher | Cat# A32731 |
| Alexa Fluor 568 goat anti-mouse secondary antibody | ThermoFisher | Cat# A11031 |
| Bacterial Strains | ||
| NEB 5-alpha competent E. coli | NEB | C2987U |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Fibronectin | EMD Millipore | Cat# FC010 |
| Alexa Fluor 568 phalloidin | ThermoFisher | Cat# A12380 |
| Alexa Fluor 647 phalloidin | ThermoFisher | Cat# A22287 |
| DAPI | ThermoFisher | Cat# D1306 |
| Hoechst | Invitrogen | Cat# H1399 |
| Y-27632 | Sigma-Aldrich | Cat# 688000 |
| Cytochalasin D | EMD Millipore | Cat# 504776 |
| PF573228 | Sigma-Aldrich | Cat# PZ0117 |
| mPEG-silane | Laysan Bio | Cat# MPEG-SIL-5000 |
| Rhodamine-labeled G-actin (Rabbit skeletal muscle) | Cytoskeleton | Cat# AR05 |
| Janelia Fluor 646 HaloTag ligand | Promega | Cat# GA1121 |
| Polyvinylpyrrolidone | Sigma-Aldrich | Cat# PVP10 |
| Critical Commercial Assays | ||
| QIAprep spin miniprep kit | Qiagen | 27106 |
| PureYield plasmid maxiprep system | Promega | A2392 |
| SE Cell Line 4D-Nucleofector X Kit L | Lonza | V4XC-1024 |
| Experimental Models: Cell Lines | ||
| MEF | Mary Beckerle Lab Huntsman Cancer Institute University of Utah | N/A |
| U2OS | ATCC | HTB-96 |
| FreeStyle 293-F | ThermoFisher | R79007 |
| Oligonucleotides | ||
| DNA primers, see Table S2 | This study | N/A |
| Recombinant DNA | ||
| mApple-F-tractin | Michael Davidson Lab Florida State University | N/A |
| Zyxin-fusionRed | Michael Davidson Lab Florida State University | N/A |
| Myosin Va (AA 1–1091) | Dharmacon | BC172485 |
| Myosin VI (AA 1–1021) | James Sellers Lab NHLBI, NIH | N/A |
| LifeAct-mApple | Mary Beckerle Lab Huntsman Cancer Institute University of Utah | N/A |
| Other plasmid DNAs, see Table S2 | This study | N/A |
| Software and Algorithms | ||
| FIJI | Schindelin et al., 2012 | https://imagej.net/Fiji |
| ChimeraX | Goddard et al., 2018 | https://www.cgl.ucsf.edu/chimerax/ |
| JalView | Waterhouse et al., 2009 | https://www.jalview.org/ |
| GraphPad Prism 8 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| Custom Python scripts computing actin and nuclear enrichments | This paper | https://github.com/alushinlab/LIM-domain |
| Custom Python scripts for patch detection and analysis | This paper | https://github.com/alushinlab/LIM-domain |
| Other | ||
| 4-well stretch chamber | Strexcell | STB-CH-4W |
| Flexcell UniFlex culture plates | Flexcell | UF-4001P |
| 50-kPa softwell 12G plate | Matrigen | 50-kPa |
| 12-kPa softwell 12G plate | Matrigen | 12-kPa |
| CultureWell reusable gasket | GraceBio | 103280 |
| NuPAGE 12% Bis-Tris gel | Invitrogen | NP0343BOX |
Also see: Table S2. Additional oligonucleotides and recombinant DNAs used in this paper. Related to the Key Resources Table.