Figure 2. Mast cell deficiency abrogates early neutrophil recruitment.

(A) Representative flow cytometry dot plots showing the frequencies of ckit⁺FcεR1⁺ mast cells in the ocular surface tissues (upper panels) and peritoneum (lower panels) of cKit^W-sh mice, relative to wild type mice. (B) Representative flow cytometry dot plots (left) and cumulative bar chart (right) showing the frequencies of CD45⁺ inflammatory cells in the corneas of cKit^W-sh mice and wild type mice at 6 hours after injury, relative to normal control mice. (C) Representative flow cytometry dot plots (left) and cumulative bar chart (right) showing the frequencies of CD11b⁺Ly6G⁺ neutrophils in the corneas of cKit^W-sh mice and wild type mice at 6 hours after injury, relative to normal mice. Representative data from two independent experiments are shown, and each experiment consisted of three to four animals. Data presented are mean ± SD (error bar). *p < 0.05, **p < 0.01, ***p < 0.001.