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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Ocul Surf. 2020 Jun 29;18(4):633–640. doi: 10.1016/j.jtos.2020.06.006

Figure 3. IL-33 activates mast cells and promotes mast cell expression of CXCL2.

Figure 3.

(A) ELISA analysis of CXCL2 levels in the supernatants of mast cells stimulated with IL-33 compared to unstimulated cells. Supernatants from cultures of mast cells stimulated with IL-33 were analyzed for mast cell activation using (B) β-hexosaminidase (C) tryptase. Supernatant from unstimulated mast cells were used as controls. (D) Representative flow cytometry histogram showing the surface expression of IL33R (ST2; White) on mast cells as compared to isotype control (Grey). Representative data from three independent experiments are shown, and each experiment was repeated three times. Data presented are mean ± SD (error bar). *p < 0.05.