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. 2020 Nov 11;11:562587. doi: 10.3389/fimmu.2020.562587

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Copyright © 2020 Leborgne, Taddeo, Freigang and Benarafa

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcription factor expression analysis of iNKT subsets indicates normal thymic iNKT17 cells in Sb1a^−/− mice. (A) Representative flow cytometry plots of iNKT cell subsets based on PLZF, T-bet and RORγt expression on CD1d-tet⁺ iNKT cells. (B) Percentages and numbers of iNKT17, 1 and 2 in thymus (B), spleen (C), lung (D), and lymph nodes (E). Percentages of total iNKT and iNKT17 Ki-67⁺ cells in indicated organs. (F) Data were from groups of 6- to 8-week-old mice matched for sex and age between genotypes. Data are from at least four independent experiments. Values for individual mice are shown with median and interquartile range.