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. 2020 Nov 11;11:562587. doi: 10.3389/fimmu.2020.562587

Figure 4.

Figure 4

Normal cytokine release following systemic or in vitro activation of iNKT cells by αGalCer in Sb1a−/− mice. (A–C) WT and Sb1a−/− mice (6–8 weeks old) were injected intravenously with αGalCer. (A) Serum cytokine levels were measured by ELISA at indicated time points. (B) Dendritic cell expression of co-stimulatory molecules CD40, CD80, and CD86 and (C) antigen presenting molecules CD1d and MHCII were measured by flow cytometry (MFI, mean fluorescence intensity). Data from three independent experiments; n = 6–12/genotype. (D) Analysis of WT and Sb1a−/− splenocytes stimulated with indicated concentrations of αGalCer. IL-17, IFNγ, and IL-4 levels were measured by ELISA in the supernatant after 48 h. Data were from groups of 6- to 8-week-old mice matched for sex and age between genotypes. Data are from four independent experiments. Values for individual mice are shown as mean and SEM.