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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: J Comp Neurol. 2020 Jun 27;529(3):481–500. doi: 10.1002/cne.24959

Figure 7. Time course of FMRP immunoreactivity changes in NM neurons at 12 and 24 hours following unilateral cochlea removal.

Figure 7.

a-c: Representative images showing FMRP immunoreactivity in the intact (a) and afferent-deprived NMs at 12 (b) and 24 hours (c). Dashed lines outline the border of the NM. a1-c1: High-magnification view of the boxes in a-c, respectively. a2-c2: NeuroTrace staining of the same regions in a1-c1. Stars indicate neurons with normal looking Nissl and FMRP staining. Arrows point to neurons exhibiting distinct FMRP puncta and reduced cytoplasmic Nissl substance. Arrowheads indicate neurons with normal Nissl stain but low FMRP immunostaining (dashed circle). d-e: Frequency histograms of z-scores of FMRP immunoreactivity in the intact and afferent-deprived NM neurons. Abbreviations: h, hour; NM, nucleus magnocellularis; OD, optical density. Scale bars = 50 μm in a (applies to a-c); 10 μm in a1 (applies to a1-c2).