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. Author manuscript; available in PMC: 2021 Aug 17.
Published in final edited form as: Angew Chem Int Ed Engl. 2020 Jul 9;59(34):14481–14489. doi: 10.1002/anie.202003500

Figure 4.

Figure 4.

Western blot analyses of total EGFR protein level in L858R/T790M BaF3 cells after cells were (A) treated in a competitive study with either DMSO, 10 μM pomalidomide or 1 μM JBJ-07-149 for 24 hours; or (B) treated in a rescue study with increasing concentrations of the NEDD8 activating enzyme inhibitor, MLN4924, for 24 hours in the presence or absence of DDC-01-163. Tubulin was used as a loading control for relative protein expression. Densitometry analysis were performed and shown as a percentage of EGFR protein left compared to DMSO control after data was normalized to tubulin.