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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2020 Oct 6;1866(1):158825. doi: 10.1016/j.bbalip.2020.158825

Figure 3. Induction of CHOP expression by AcLDL after Soat1 editing in DBA/2 embryonic stem cell derived macrophages.

Figure 3.

DBA/2 wildtype or Soat1 Δ DBA/2 ESDM were incubated with 50 μg/ml AcLDL without or with the addition 30 μg/ml ACAT inhibitor for 16–24 hrs. A. Free, esterified, and total cholesterol levels, normalized to total cell protein. B. Ddit3 mRNA levels measured by qPCR. C. CHOP protein expression was observed by western blot, with β-actin as an internal control. AcLDL, acetylated low-density lipoprotein; ACATi, ACAT inhibitor. Statistical difference of <0.05 was determined by ANOVA with Tukey’s multiple comparison test and is shown by different letters above the bars.