Table 1.

Designed primers of quantitative real-time PCR.

Gene name	Forward primer sequence	Reverse primer sequence	Product size/bp	Annealing temperature/°C
AcSPS1	CTATCAATGACAAGAAGGGCGAAAA	GCAACGGTGAGCCTGAATCCT	158	63.6
AcSPS2	ATGCTTTTCACTGGTCACTCACT	CATCATACAGACGCCATTGCTC	199	58.2
AcSPS3	TTCTGAAGTTGGTCCTTTTGGG	TCGTCTTCAGCAACTTGTCCTC	165	61.2
AcSPS4	ACGAGCACCAAGCAGGAGATT	CCACAACATTGCTGAAGTCCATA	169	61.3
AcSPS5	GGAGAAGGAAAAGGGTGATGC	CCTGACCTCCAGTGTCTGAATC	191	58.4
AcSUS1	AACTTGGGATTACTCTGGGAACT	ATTTCTTGGTATGTGCTGGTGAT	175	58.5
AcSUS2	GGTGGCTTACAGAAGGCTCAG	CCACTGCCTAAACCTTTGCTC	195	59.2
AcSUS3	GTTGCTTTGGCAGTGAGGAGG	GGTTAGGCGACGACTTGAGAAA	198	61.5
AcSUS4	TCAAAGAATACAACTTGGATGGC	CAAGTGGCAAATGTTGGAAGC	187	59.4
AcSUS5	TTGGGCTATCCTGACACTGGT	CAATGAGAATGCGTGGAATGA	124	59.4
AcSUS6	ACACTGGTGGTCAGGTGGTTT	GCGAATGTTCTGCTCCGTAAA	189	59.8
AcSUS7	ATCAAAGCCCATTATCTTCTCCA	TGACTTCTTCACATCATTGTAACCC	145	60.1