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. 2020 Nov 24;11:5969. doi: 10.1038/s41467-020-19803-9

Fig. 1. Late fed-state transcriptional repression of lipogenic genes is dependent on both FGF15 and SHP.

Fig. 1

Mice were fasted overnight and refed normal chow for the indicated times. a Relative levels of pre-mRNAs of lipogenic genes in the livers of C57BL/6 mice measured by RT-qPCR using primers spanning the intron 1 and exon 1 boundaries and b mRNA levels of these genes. c–f Relative levels of hepatic Fasn pre-mRNA (c, left), mRNA (c, right), and protein (e) in FGF15-KO mice or control littermates. Relative levels of hepatic Fasn pre-mRNA (d, left), mRNA (d, right), and protein (f) in SHP-KO mice or control C57BL/6. Data presented as mean values ± Standard Deviation (SD) are shown (a, b, n = 5 mice; c, n = 4 for 2 h, n = 5 for other times, mice; d, n = 6 mice; e, f, n = 5 for 0 time, FGF15-KO mice, n = 3 for other times, mice), and statistical significance was determined by (a, b) one-way ANOVA with the Tukey post-test or (c–f) two-tailed Student’s t-test. * P < 0.05, ** P < 0.01, ns, statistically not significant.