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. 2020 Sep 16;123(11):1644–1655. doi: 10.1038/s41416-020-01040-y

Fig. 2. Detection of calcification in vitro.

Fig. 2

a Calcification of breast cancer cells was measured with alizarin red S staining at different time points after OC administration. Scale bars = 200 µm. b The ALP activity was measured at different time points after addition of OC in MDA-MB-468 and MCF-7 cells. c Western blotting of the calcification-related genes at the indicated time upon induction with OC. d mRNA levels of the corresponding molecules in MDA-MB-468 and MCF-7 cells were detected using quantitative RT-PCR and expressed as a ratio to β-actin. *P < 0.05, **P < 0.01, ***P < 0.001, NS not significant, determined by unpaired Student’s t test and one-way ANOVA with Bonferroni post-test correction. Data are shown as mean ± SD, and are representative of three independent experiments with similar results.