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. 2020 Sep 16;123(11):1644–1655. doi: 10.1038/s41416-020-01040-y

Fig. 4. Minor alterations in glycolysis metabolism of breast cancer cells during osteogenic differentiation.

Fig. 4

Western blotting (a) and quantitative real-time (qRT)-PCR (b) were conducted to evaluate the expressions of selective glycolysis-related genes (e.g., HK I, HK II and PFKP) in MDA-MB-468 and MCF-7 cells at different time points after OC treatment. c Lactate dehydrogenase (LDH) activity in the cells was measured during 4 days of OC treatment. The expression of LDHA in the cells was assessed by western blotting (d) and qRT-PCR (e). f Seahorse analysis of extracellular acidification rate (ECAR) in MDA-MB-468 cells at specific time points induced by OC. g ECAR of non-glycolysis, glycolysis, glycolytic capacity and the glycolytic reserve was analysed. *P < 0.05, **P < 0.01, NS not significant, determined by unpaired Student’s t test and one-way ANOVA with Bonferroni post-test correction. Data are shown as mean ± SD, and they represent three independent experiments with similar results.