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. 2020 Nov 24;10:20460. doi: 10.1038/s41598-020-77452-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Morphological characterization of the alveolar mucosa model by confocal and transmission electron microscopy (TEM). (a) The cell junction protein zona occludin 1 (ZO1) (b) lamellar bodies, (c) surfactant protein C (SPC) and (d) epithelial sodium channel (ENaC). Nucleus is stained in blue. Bar scale: 50 µm; (e) Representative TEM image of the alveolar type II cells in air–liquid interface (2 weeks) showing microvilli (MV), lipid bodies (LB), desmosome (D), and tight junction (TJ) Bar scale: 2 µm. The microscopic images are representative of alveolar mucosa model developed at air–liquid interface (2 weeks) from NCl-H441 cells.