Figure 1.
(A) Schematic representation of bioassay-guided isolation of β-sitosterol from the chloroform extract of Corchorus capsularis L. leaves. (B) Determination of the antipromastigote activity of fractions (F1–F13). L. donovani promastigotes (1 × 107/ml) were treated individually with 13 fractions (F1–F13) at doses ranging from 0 to 200 µg/ml for 48 h, and the MTT assay was performed. F4 was found to be most active (IC50 = 17.7 ± 0.43 µg/ml), denoted by the black line, whereas the F11 and F13 fractions, denoted by dotted lines, exhibited IC50 values > 200 µg/ml. The results are expressed as the mean ± S.E. from three independent experiments.