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. 2020 Nov 24;10:20440. doi: 10.1038/s41598-020-77066-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Measurement of intracellular non-protein thiols in promastigotes. Promastigotes (1 × 10⁷/ml) were treated with β-sitosterol_CCL, and fluorescence intensity was measured after CMFDA staining at various time points, i.e., 3 h, 6 h, 12 h, and 24 h. (A) The bar graph depicts the MFI values of three independent experiments, and statistical significance was determined with respect to the control by using a t-test, where **p < 0.01 and ****p < 0.0001 were considered statistically significant. (B) Histograms depicting the reduction of MFI in β-sitosterol_CCL-treated parasites compared to the control at every time point. Data were acquired in a BD FACSCalibur flow cytometer and analysed in Flowing software (https://www.flowingsoftware.com), version 2.5.1, Finland.