Table 2.
Brief summary of different SELEX methods and the principles developed with the year of the invention
| S. no. | Year of invention | SELEX technique | Brief principle | References |
|---|---|---|---|---|
| 1 | 1992 | Negative SELEX | Exclusion of non-specific aptamers by mixing the aptamer library with a matrix | Ellington and Szostak (1992) |
| 2 | 1994 | Counter SELEX | Removal of cross-reactive species by mixing with closely related species | Jenison (1994) |
| 3 | 1997 | Genomic SELEX | An easy SELEX method that can identify the genomic sequence binding motif of the organism itself | Singer et al. (1997) |
| 4 | 1997 | In vivo SELEX | Aptamer selection under in vivo conditions made within the cell | Coulter et al. (1997) |
| 5 | 1998 | Chimeric SELEX | Preparation of recombinant aptamers that binds with two different targets | Burke and Willis (1998) |
| 6 | 1999 | Multi-stage SELEX | Aptamers designated against dissimilar targets fused and reselected against such targets through a multi-step process | Wu and Curran (1999) |
| 7 | 1999 | Cell-SELEX | Aptamer preparation targeting the markers of the whole cell | Homann and Goringer (1999) |
| 8 | 2000 | Indirect SELEX | Preparation of special aptamers directed to bind with the native protein in the presence of a metal ions | Kawakami et al. (2000) |
| 9 | 2000 | Photo-SELEX | Light-sensitive nucleotide insertion to design the aptamers targeted directly after UV irradiation | Golden et al. (2000) |
| 10 | 2001 | Toggle SELEX | The synthesis of cross-reactive aptamers while targeting toggling during selective preparation | Bianchini et al. (2001), White et al. (2001) |
| 11 | 2003 | Tailored SELEX | The short and primer free aptamer selection sequences using cleavable primer-hybridization sites | Vater et al. (2003) |
| 12 | 2004 | CE-SELEX | Aptamer selection by the application of electrophoretic mobility shift standards | Mendonsa and Bowser (2004) |
| 13 | 2005 | FluMag-SELEX | The use of fluorescence tagged targets that allows cleaner aptamer selection | Stoltenburg et al. (2005) |
| 14 | 2006 | TECS-SELEX | Recombinant proteins expressed on modified cells used for aptamer selection | Ohuchi et al. (2006) |
| 15 | 2006 | NECEEM (non-SELEX) | Aptamer selection based on non-equilibrium electrophoresis | Berezovski et al. (2006) |
| 16 | 2007 | Nano selection-based SELEX | Atomic force and fluorescence microscopy integration to purify aptamer in a single step | Peng et al. (2007) |
| 17 | 2007 | MonoLEX | Specific sequence selection through chromatography column fragmentation and pyrosequencing | Nitsche et al. (2007) |
| 18 | 2010 | Microfluidic SELEX | The purification of aptamers with the help of microfluidic chips integrating at different steps on a single surface | Cho et al. (2010); Huang et al. (2010) |
| 19 | 2011 | High-throughput SELEX | The evaluation of high-throughput DNA sequencing by the combination of bioinformatic analysis | Hoon et al. (2011) |
| 20 | 2014 | Particle display SELEX | Flow cytometry-based selection of aptamer particles | Wang et al. (2014a, b, c, d) |
| 21 | 2015 | Hi-fidelity SELEX | Digital PCR used to enhance the SELEX progress by the inclusion of fixed sequence blocking elements | Ouellet et al. (2015) |
| 22 | 2016 | Isogenic cell SELEX | The selection of overexpressed isogenic cell line as SELEX target and downregulating it by micro RNA-mediated silencing for the counter selection | Takahashi et al. (2016) |
CE-SELEX capillary electrophoresis SELEX, FluMag fluorescent/magnetic beads, TECS target expressed on cell surface, NECEEM non-equilibrium capillary electrophoresis of equilibrium mixtures, MonoLEX affinity chromatography SELEX