FIGURE 2.

Microcalorimetry analysis of MRSA ATCC 43300 (upper row) and MSSA ATCC 29213 (bottom row) biofilms after staggered application of Sb-1 phage for 24 h followed by 24 h exposure to sub-MBBC concentrations of antibiotics. Each curve shows the heat produced by viable bacteria present in the biofilm after phage–antibiotic treatment. Numbers represent concentrations (in μg/ml) of vancomycin (VAN), daptomycin (DAP), fosfomycin (FOF), gentamicin (GEN), rifampin (RIF), flucloxacillin (FLU), and cefazolin (CFZ). Circled values represent the MBBC, defined as the lowest antimicrobial concentration leading to absence of bacterial regrowth after 48 h. GC, growth control; NC, negative control. Data of a representative experiment are reported.