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. 2020 Nov 24;10:20444. doi: 10.1038/s41598-020-77169-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Endometabolic changes of Phaeocystis pouchetii AJ01 during growth in laboratory batch cultures (n = 4). Separation is based on canonical analysis of principal coordinates (CAP); axis eigenvalues are given in parentheses. (a) Score plot of the growth phases: exponential phase ('exp', bright green circle), early stationary phase ('e.stat', green triangle), late stationary phase ('l.stat', dark green square). (b) Loading plot (scaled to score plot) of correlated endometabolites (> 0.708) depicted with their identifier as tabulated in the Supplementary Table S6. (c) Heat map of correlated endometabolites (analyte peak area/peak sum) indicating low (blue) or high (red) relative concentrations in the growth phases. Metabolites and samples are clustered hierarchically for better visualization using the Pearson distance measure and Ward cluster algorithm.