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. 2020 Nov 24;10:20487. doi: 10.1038/s41598-020-77514-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Adhesion to collagen is increased in Sdc3^−/− MSCs. MSCs were isolated from wild type (n = 6) and Sdc3^−/− (n = 6) mice and seeded onto several different substrates in complete expansion medium (CEM). After 60 min the average percentage of wild type (WT) and Sdc3^−/− MSCs adhered to each substrate was calculated: (a) Non-coated, (b) Collagen type I, (c) Fibronectin and (d) Laminin. (e) A scratch assay was performed on MSCs cultured in serum free medium (SFM), reduced serum content medium (rCEM) or full serum content medium (fCEM) and the average percentage of gap closure is shown. (f) Cell migration towards specific chemoattractants was measured using a transwell assay. The number of migrated cells was calculated after 15 h of incubation to each chemoattractant. PTN, pleiotrophin. Data presented as mean ± standard deviation; *p < 0.05.