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. 2020 Nov 24;10:20493. doi: 10.1038/s41598-020-77568-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Generation and characterization of endothelial-specific YY1 deficient mice for the tumor angiogenesis. (A) Schematic diagram for the endothelial cell specific deletion of YY1 in mice (Ve-Cad-CreER^T2; YY1^flox/flox , YY1^iΔEC ) and the strategy of the tumor angiogenesis study. The tumor was induced by melanoma B16-F10 cells (5 × 10⁶ cells per mouse) with subcutaneously transplanted into 8-week-old WT or YY1^i∆EC mice. (B) PCR analysis for the genotyping of Ve-Cad-CreER^T2; YY1^flox/flox mice (YY1^iΔEC) and YY1^flox/flox (WT) mice. (C) Immunofluorescence image of endothelial cell marker VE-Cadherin in lung endothelial cells isolated from YY1^iΔEC mice. Nuclei were labeled by DAPI (blue) (Left panel) and Western blot analysis of endothelial YY1 expression in mouse lung endothelial cells isolated from WT and YY1^iΔEC mice (n = 3) (Right panel). (D) Dual immunostaining analysis of YY1 (red) and endothelial cell marker CD31 (green) in melanoma tumor tissues isolated from WT and YY1^iΔEC mice, (n = 7). Scale bars: 20 μm.