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. 2020 Nov 11;11:592553. doi: 10.3389/fimmu.2020.592553

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Copyright © 2020 Binder, Sellberg, Cvetkovski, Berglund and Berglund

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Target antigen expression on PBMC. Data is displayed as the average median fluorescent intensity (MFI) ± SD (N = 5). PBMC were stained using the following drugs: siplizumab (Target antigen CD2), Alemtuzumab (CD52) and rATG (Thymocyte antigens). After removal of unbound antibody, samples were stained with a secondary anti-human IgG antibody and MFI for the secondary antibody was recorded, representing the expression of the respective transplant antigens. Tcm, central memory T cells; Tem, effector memory T cells; Temra, effector-memory CD45RA⁺ T cells; rTreg, resting regulatory T cells; nsTreg, non-suppressive Treg; aTreg, activated Treg; CD56^Bright, CD56^bright NK cells; CD56^Dim, CD56 ^dim NK cells; CD56^neg CD16⁺, CD56⁻ CD16⁺ NK cells.