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. 2020 Nov 11;8:593508. doi: 10.3389/fcell.2020.593508

FIGURE 6.

FIGURE 6

Modulation of hypoxia inducible factor-1α (HIF-1α) pathway induced by WNT7A silencing upon physical and pharmacological hypoxia. (A,B) Western blot analysis (A) and relative quantification (B) of HIF-1α nuclear localization in wild type (WT) and WNT7A-silenced murine myoblasts (WNT7A-KO) treated under normoxia, hypoxia, FG-4592 or IOX2. The nuclear marker Lamin A/C was used as the housekeeper (n = 4 for Normoxia, n = 3 for Hypoxia, IOX2 and FG-4592). (C) qPCR analysis of HIF-1α target genes, VEGF, PHD2, and PHD3 in WT and WNT7A-silenced murine myoblasts (WNT7A-KO) upon physical and pharmacological hypoxia (n = 4 for Normoxia, n = 3 for Hypoxia, IOX2 and FG-4592). Data information: all data represent mean ± SD. For western blot analysis of HIF-1α *p < 0.05 (Student’s t-test). For qPCR analysis of HIF-1α target genes *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (ordinary one-way ANOVA).