FIGURE 8.

Modulation of MyoD nuclear translocation and myosin heavy chain (MHC) expression in wild type (WT) and WNT7A-silenced murine myoblasts upon physical and pharmacological hypoxia. (A,B) Western blot analysis (A) and relative quantification (B) of MyoD nuclear localization were tested after 24 h preconditioning under physical or chemical hypoxia in WT and WNT7A-KO. The nuclear marker Lamin A/C was used as the housekeeper (n = 3). (C,D) Western blot analysis (C) and relative quantification (D) of MHC were measured at the end of the skeletal muscle differentiation in WT and WNT7A-KO. The early endosomal antigen 1a (EE1a) was used as the housekeeper (n = 3). Data information: all data represent mean ± SD. *p < 0.05, **p < 0.01, ****p < 0.0001 (Student’s t-test).