Figure 3.

3D-dynamic perfusion-based immune competent systems to study extracellular matrix (ECM). Development of 3D-dynamic immune competent models to study ECM properties in vitro. (A) whole liver decellularization generates an acellular scaffold with maintained vasculature and ECM network. The resulting liver scaffold can be placed in a bioreactor which allows continuous circular perfusion of immune cells through the decellularized liver vasculature, facilitating immune cell:ECM interaction and monitoring of cellular phenotypes. (B) segments of liver tissue can be obtained from patient liver tissue and decellularized to generate acellular liver segments. These can be cultured in a multi-well bioreactor which supports perfusion of immune cells. The multi-well set up allows for direct comparison between eg healthy vs diseased tissue perfused with the same immune cells. Schematic was created using BioRender (https://app.biorender.com).