Fig. 2.

Inhibited NEAT1 or elevated miR-129-5p inhibits the elevated lipid metabolism and restrains inflammation reaction in ethanol-stimulated AML-12 cells. A, impacts of different concentrations of ethanol on AML-12 cell viability were evaluated by MTT assay; B, the expression of NEAT1, miR-129-5p, and SOCS2 in AML-12 cells in the ethanol untreated group and the ethanol treated group; C, the expression of NEAT1, miR-129-5p, and SOCS2 in AML-12 cells that have been treated with ethanol after transfection; D, contents of TC and TG in AML-12 cells that have been treated with ethanol after transfection; E, contents of TNF-α, IL-1β, and IL-6 in AML-12 cells that have been treated with ethanol after transfection; a *P < 0.05 vs the AML-12 cells that have not been stimulated by ethanol; b *P < 0.05 vs the untreated group; c–e *P < 0.05 vs the siRNA-NC group, ^#P < 0.05 vs the mimic-NC group, ^&P < 0.05 vs the oe-NEAT1 + mimic-NC group; N = 3, the measurement data were expressed as mean ± standard deviation, one-way ANOVA was used for comparisons among multiple groups, and Tukey’s post hoc test was used for pairwise comparisons after one-way ANOVA