Fig. 6.

MiR-129-5p particularly binds with NEAT1, and SOCS2 is targeted by miR-129-5p. a binding sites of NEAT1 and miR-129-5p were predicted by https://starbase.sysu.edu.cn/; b binding relation between NEAT1 and miR-129-5p was confirmed by dual luciferase reporter gene assay; c effect of miR-129-5p on enrichment of NEAT1 was measured by RNA pull-down assay; d target relation between miR-129-5p and SOCS2 was predicted by https://www.targetscan.org/vert_72/; e target relation between miR-129-5p and SOCS2 was confirmed by dual luciferase reporter gene assay; the measurement data were expressed as mean ± standard deviation, the unpaired t-test was performed for comparisons between two groups, one-way ANOVA was used for comparisons among multiple groups, and Tukey’s post hoc test was used for pairwise comparisons after one-way ANOVA. The experiment was repeated for 3 times