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. 2020 Nov 23;10:134. doi: 10.1186/s13578-020-00497-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Both CXCR4 and CXCR7 are essential for cell migration. a Wild-type (W) and each HeLa cell clone lacking the receptors (7KO #1,2,3 and 4KO #1,2) were seeded into 96-well plates. The CCK-8 assay was performed using different plates each day. Values are means ± SDs. b, c Migration assay using HeLa cells. Wild-type (W) and knock-out cells infected with CXCR7 (7KO/ CXCR7) or CXCR4 (4KO/ CXCR4) were placed in the upper wells of transwell plates. 50 ng/ml of SDF-1α was added to serum-free media in the lower wells (NT: no treatment). After 24 h, migrated cells in the lower wells were stained and counted under an inverted microscope. c The average number of migrated cells (four different microscopic fields) is shown for the five groups. d Migration assay with U937 cells. CXCR7 knock-out U937 cells were re-introduced CXCR7 by infection (V: empty plasmid). Cells were placed in the upper wells, with the lower well containing increasing concentration of SDF-1α in serum-free medium. After 6 h, migrated cells into the media of the bottom wells were counted using a hemocytometer. Cell numbers are averages of migrated cells from three different wells. Results are the average of three independent experiments. Values are presented as the mean ± SD. **p < 0.01, **p < 0.001.